| 1. | Spleen cells from negative control mice did not proliferate upon restimulation 3和3刁,而生理盐水对照组增汛不显著。 |
| 2. | Effects of dihydroartemisinin on apoptosis of spleen cells in rats infected with pneumocystis carinii pneumonia 双氢青蒿素对卡氏肺孢子虫超微结构的影响 |
| 3. | Effects of treatment with dihydroartemisinin on no in the supernatant of spleen cells in rats infected with pneumocystis carinii pneumonia 经双氢青蒿素治疗后卡氏肺孢子虫肺炎大鼠的肺部病理学变化 |
| 4. | Sp2 / 0 myeloma cells are fused with spleen cells of balb / c mice ( the ratio is 1 : 10 ) immunized with at - by routine method . positive clones are detected by elisa Sp2 / 0细胞和免疫脾细胞按1 : 10比例融合,融合剂peg分子量为1500 ,阳性克隆筛选采用三步进行。 |
| 5. | Ifny levels in culture supernatants of spleen cells from balb / c mice immunized with mpt64 and bcg were 1024ng / l and 1470 ng / l respectively . but ifny levels in that of saline immunized was below 80ng / l 纠果门”见mpt64蛋白抡疫刁鼠和bcg免疫小鼠脾细胞分泌的ifny含量分别达到1024ng l和1470ng几。 |
| 6. | The response of normal rats and only tbi conditioned rats spleen cells to balb / c and c57bl / 6 mice spleen cells were all violent , the values of si were 2 . 22 + 0 . 26 , 2 . 28 + 0 . 27 , 2 . 30 + 0 . 15 , 2 . 18 + 0 . 31 正常未经处理大鼠及只经tbi处理大鼠的脾细胞对balwc和c57bl历’ j ’鼠的脾细胞均有强烈增殖反应, si值分别为2 |
| 7. | In conclusion , so2 can not only affect respiratory system , but also other organs such as spleen , liver , thymus on their histological structures and every organ performs very differently under so2 exposure . so2 derivatives has cytotoxicity both on mouse spleen cells and human embryo lung fibroblasts 说明二氧化硫代谢衍生物对人胚肺成纤维细胞和小鼠脾细胞都有一定毒性,并且在一定范围内对小鼠脾细胞具有一定的凋亡诱导作用。 |
| 8. | ( 5 ) effects on mouse cerebrum cortex of so2 challenge : we found no pathological changes of mouse cerebrum cortex challenged by so2 by he staining and tem observation , in vitro tests of so2 derivatives challenge showed that : ( 1 ) so2 derivatives has significant toxicity on mouse spleen cells , the lc50 of 4h and 24h exposure is 1 . 1293 mmol / l and 1 . 3777mmol / l respectively 二氧化硫代谢衍生物体外实验结果表明: ( l )二氧化硫代谢衍生物对小鼠脾细胞具有明显的细胞毒性作用,染毒4小时和24小时细胞的半数存活抑制浓度lcs 。分别为1 . 12932r以mol几和1 . 37777 ~ ol几。 |
| 9. | 5 in vitro specific killing experiments showed that spleen cells induced by pi , p3 and p4 , which contained higher proportion of cdg + cells and generated better ctl activity , could be introduced to in vivo experiment against hcv 6 we successfully setup a hcv structural gene in vitro cell - transfection system sp2 / 0 - hcv , through which also setup a murine model with subcutaneous transplanting tumor of hcv 将诱导的5种v特异性c l ,在体外进了丁特异性杀伤实验,证一实,出n 、 p3和p4三条多肽诱导的脾纠泡含较高比例的cd 。纠1巾d ,叫产上较好的ctl杀伤活性,可以用作t细胞疫苗进fi体内抗hcv实验。 |
| 10. | Meq protein , highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro . the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef ) 利用通过杆状病毒载体在昆虫细胞系sfg上高度表达的meq蛋白产物免疫balb / c小鼠,然后收获其免疫脾细胞并与肿瘤细胞系spz / 0通过peg于体外融合;获得的杂交瘤细胞被克隆并通过与mdv感染的鸡胚成纤维细胞( cef )做免疫荧光试验( fa ) ,进行其分泌抗meq单克隆抗体( mcab )能力的筛选。 |